内容紹介
Survival Benefit by Combined Administration of Cyclophosphamide, Lentinula edodes Mycelia Extract(LEM), and Ganoderma lucidum Mycelia Extract(MAK)in S1018B10 Tumor-Bearing Mice
Summary
Cyclophosphamide(CY)was intraperitoneally administered once a week to C57BL/10 mice that had received Rous sarcoma virus(RSV)-induced S1018B10 syngeneic tumor transplantation and in whom tumor diameter exceeded 4.5 mm. Survival was prolonged in a group of mice that also received a mixture of LEM and MAK orally. When splenic cells were cultured under mitomycin C-treated S1018B10 stimulation and the S1018B10-directed cell killing ability was examined, the effector cells were found to be F4/80- DC/Mφ cells. Flow cytometric analysis showed that the proportion of F4/80- DC/Mφ cells in the splenic cell culture of the CY+LEM+MAK treatment group was higher than that in the untreated group. The ratio of F4/80+ CD8a+ cells in the CY+LEM+MAK treatment group was lower than that in the untreated group.
要旨
C57BL/10マウスにRous sarcoma virus(RSV)誘発同系腫瘍のS1018B10を移植して腫瘍径4.5 mmを超えてからcyclophosphamide(CY)を週1回腹腔投与し,Lentinula edodes mycelia extract(LEM)とGanoderma lucidum mycelia extract(MAK)の混合物を経口投与すると,無治療群に比べて延命した。脾細胞をmitomycin C処理したS1018B10刺激下に培養してS1018B10に対する殺細胞能力を調べると,effector細胞はF4/80-のDC/Mφ系細胞であった。フローサイトメトリー解析でCY+LEM+MAK治療群の脾細胞培養中のF4/80-DC/Mφ系細胞の比率は無治療群よりも上昇していた。CY+LEM+MAK治療群のF4/80+CD8a+細胞比率は無治療群よりも低下していた。
目次
Summary
Cyclophosphamide(CY)was intraperitoneally administered once a week to C57BL/10 mice that had received Rous sarcoma virus(RSV)-induced S1018B10 syngeneic tumor transplantation and in whom tumor diameter exceeded 4.5 mm. Survival was prolonged in a group of mice that also received a mixture of LEM and MAK orally. When splenic cells were cultured under mitomycin C-treated S1018B10 stimulation and the S1018B10-directed cell killing ability was examined, the effector cells were found to be F4/80- DC/Mφ cells. Flow cytometric analysis showed that the proportion of F4/80- DC/Mφ cells in the splenic cell culture of the CY+LEM+MAK treatment group was higher than that in the untreated group. The ratio of F4/80+ CD8a+ cells in the CY+LEM+MAK treatment group was lower than that in the untreated group.
要旨
C57BL/10マウスにRous sarcoma virus(RSV)誘発同系腫瘍のS1018B10を移植して腫瘍径4.5 mmを超えてからcyclophosphamide(CY)を週1回腹腔投与し,Lentinula edodes mycelia extract(LEM)とGanoderma lucidum mycelia extract(MAK)の混合物を経口投与すると,無治療群に比べて延命した。脾細胞をmitomycin C処理したS1018B10刺激下に培養してS1018B10に対する殺細胞能力を調べると,effector細胞はF4/80-のDC/Mφ系細胞であった。フローサイトメトリー解析でCY+LEM+MAK治療群の脾細胞培養中のF4/80-DC/Mφ系細胞の比率は無治療群よりも上昇していた。CY+LEM+MAK治療群のF4/80+CD8a+細胞比率は無治療群よりも低下していた。